Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Total p21 CiP1 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Total p21 CiP1 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-ATM (Ser1981) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-ATM (Ser1981) Detection Kit, 50,000 Assay Points
Molecular Devices Filter Lens, 562 nm, EMax and VMax Compatible
Replacement optical filter lens providing a 562 nm wavelength selection for microplate reader measurements. It is compatible with Molecular Devices EMax and VMax microplate readers and installs in the instrument filter assembly to enable detection at 562 nm.
- Provides 562 nm wavelength selection for optical measurements.
- Compatible with EMax and VMax microplate readers.
- Supports fluorescence and absorbance assays requiring 562 nm detection.
- Replacement lens for instrument filter assemblies.
- Available on request; contact supplier for availability.
Molecular Devices Time-Resolved Emission Filter, 616 nm, 10 nm Bandwidth
This Molecular Devices time-resolved emission filter provides a 616 nm center wavelength with a 10 nm bandwidth for time-resolved fluorescence (TRF) and TR-FRET detection in multi-mode microplate readers. It is intended to isolate emission bands and improve signal selectivity in time-resolved assays.
- Optimized for time-resolved fluorescence and TR-FRET assays.
- 616 nm center wavelength, 10 nm bandwidth.
- Emission filter configured for use in microplate reader filter holders.
- Improves emission selectivity and reduces background signal.
- Referenced in instrument application notes for optimized TR-FRET settings.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-SMAD4 (Thr277) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-SMAD4 (Thr277) Detection Kit, 10,000 Assay Points
Perkin Elmer US LLC Micro ATR Objective for the Multiscope Systems
This accessory offers transmission, reflectance, and visible viewing modes all in one device; no realignment or extra equipment required.
Revvity Health Sciences Inc HTRF XIAP BIR3 Binding Kit Red Ligand, 1 Vial
HTRF XIAP BIR3 Binding Kit Red Ligand, 1 Vial
Revvity Health Sciences Inc HTRF Human Phospho-Cyclin D1 (Thr286) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-Cyclin D1 (Thr286) Detection Kit, 10,000 Assay Points
Electron Microscopy Sciences MAC X-Ray Fluorescence Single Standard Nd
These standards are supplied as 1/4" (33 mm) diameter pressed pellets or metal foils, and are prepared from selected high purity elements and compounds to ensure interference free-spectra. They are suitable for use by experienced spectroscopists as well as staff training. Each of the pressed pellets is supported by a thin-walled aluminum cup which is protected from damage during handling and minimizes the risk of touching the standard materials (some of which are harmful).
Perkin Elmer US LLC Sample Capillary Adapter for GemCone and GemTip Nebulizers
This Capillary Adapter, or Gland Nut, is included in the GemTip High-Sensitivity Plastic Body Nebulizer Complete Assembly for AAnalyst (N0370393) and the Universal GemTip Corrosion-Resistant Plastic Body Nebulizer Complete Assembly for AAnalyst (N0370394). It is used in the GemCone Low Flow Nebulizer (N0770358) and the GemCone High Dissolved Solids Nebulizer (N0690670).
Revvity Health Sciences Inc HTRF Human Phospho-TYK2 (Tyr1054/1055) Detection Kit, Control Lysate
HTRF Human Phospho-TYK2 (Tyr1054/1055) Detection Kit, Control Lysate
Molecular Devices SpectraDrop Cover Slides, 4 μL, 1.0 mm Path
Molecular Devices SpectraDrop Micro-Volume cover slide refills provide replacement cover slides for low-volume absorbance and fluorescence measurements. Each pack contains three 4 μL cover slides with a 1.0 mm path length, designed to restore and extend micro-volume workflows while minimizing sample consumption.
- Pack contains three 4 μL cover slides.
- Path length 1.0 mm.
- Enables measurements with sample volumes as low as 2 μL.
- Designed for micro-volume absorbance and fluorescence measurements.
- Helps reduce sample consumption and increase throughput.
Molecular Devices Absorbance Filter, 420 nm, 8 nm bandwidth, FilterMax
Molecular Devices FilterMax absorbance filter designed for fixed-wavelength absorbance assays. The filter provides narrowband transmission centered at 420 nm with approximately 8 nm full width at half maximum and is supplied as an accessory for FilterMax microplate readers.
- Provides narrowband transmission centered at 420 nm.
- Approximately 8 nm full width at half maximum for high wavelength specificity.
- Optimized for absorbance assays on compatible multimode readers.
- Supplied as a replaceable accessory for FilterMax F3 and F5 series readers.
Electron Microscopy Sciences USAF Test Chart R75P Positive Image
USAF resolution charts are recognized the world over as a universal standard for testing the vertical and horizontal resolution of imaging systems. Each element on the chart comprises three vertical bars and three horizontal bars, and the detail on these slides is as fine as 0.78microns (644 line-pairs per mm). The resolution of the imaging system is normally specified as the Group and Element of the finest bars that can be clearly defined. Sub Micron smallest feature size - bar width 0.00078mm [0.78 um].
Type: Positive Image
Use: Groups 0/1 to 9/3
Revvity Health Sciences Inc HTRF High Performance Human IL-6 Detection Kit, 100,000 Assay Points
HTRF High Performance Human IL-6 Detection Kit, 100,000 Assay Points