Microplate Readers and Reader Accessories

Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.

The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.

In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.

Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.

Features may include:

Monochromator- or filter-based wavelength selection
Visible only or UV/Vis spectrum, fluorescence, and luminescence
Multiple photomultiplier tubes (PMTs) for simultaneous assays
Temperature and CO₂/O₂controls
Modular design for adding functionality

Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.

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241

Revvity Health Sciences Inc HTRF Human & Mouse Total CDK5 Detection Kit, Control Lysate

HTRF Human & Mouse Total CDK5 Detection Kit, Control Lysate

Pricing & Availability

242

Revvity Health Sciences Inc HTRF Human Phospho-ATM (Ser1981) Detection Kit, 50,000 Assay Points

HTRF Human Phospho-ATM (Ser1981) Detection Kit, 50,000 Assay Points

Pricing & Availability

243

Molecular Devices Microplate Reader, Multi-Mode, 8-Channel, 2 to 45 °C

The FlexStation 3 Multi-Mode Microplate Reader is a benchtop multimode instrument for kinetic and endpoint assays with integrated liquid handling and temperature control. It measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence using monochromators for flexible wavelength selection.

Integrated 8-channel pipettor for timed reagent additions.
Supports 6, 12, 24, 48, 96, and 384 well plate formats.
Detection modes include absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence.
Monochromators tunable in 1.0 nm increments for flexible wavelength selection.
Wavelength coverage suitable for absorbance and fluorescence assays.
Temperature control from 2°C above ambient to 45°C for incubated assays.
Suitable for kinetic assays, compound additions, and high-throughput workflows.