Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Total FLT3 Detection Kit, 50,000 Assay Points
HTRF Human Total FLT3 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-Cofilin (Ser3 & Total) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-Cofilin (Ser3 & Total) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total CDK9 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total CDK9 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total ATF4 Detection Kit, 50,000 Assay Points
HTRF Human Total ATF4 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc AlphaScreen mAb Anti-DIG Donor Beads, 5 mg
AlphaScreen mAb Anti-DIG Donor Beads, 5 mg
Revvity Health Sciences Inc HTRF Human & Mouse Total CREBBP Detection Kit, 500 Assay Points
HTRF Human & Mouse Total CREBBP Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human XBP1s Detection Kit, 50,000 Assay Points
HTRF Human XBP1s Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF cIAP1 BIR2 Binding Kit, 100,000 Assay Points
HTRF cIAP1 BIR2 Binding Kit, 100,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Lysis Buffer B (5X), 100 mL
AlphaLISA SureFire Ultra Lysis Buffer B (5X), 100 mL
Alkali Scientific ASI™ 6-Well Confocal Plate (35m), Clear, Adhesion Type, Sterile, 4/Cs, PS/Glass, Non-Treated (Non-Charged/ Hydrophobic), 100/Cs, 22 x 22 x 0.13mm, Hole 3.14mm
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products: Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy ▪ Bottom materials: Glass/FLux (no surface treatment for glass bottom)▪ Dish color: Clear▪ Dish size: 35 Ø▪ Plate size: 6well Plate▪ Hole size: 13 Ø / 20 Ø / 25Ø▪ Non – pyrogenic▪ Non – cytotoxic▪ DNase / RNase – free▪ Human DNA – free
Molecular Devices Photometric Filter, Absorbance 590 nm, FilterMax Compatible
The AD 340 photometric filter provides a 590 nm center wavelength bandpass for absorbance (photometric) measurements in multimode microplate readers. Supplied as an accessory by Molecular Devices (MPN 6590-0023), the filter installs in a reader's filter holder to enable targeted optical selection for absorbance assays and routine photometric measurements.
- Central wavelength 590 nm for absorbance measurements.
- Designed for photometric microplate assays.
- Installs in a standard filter holder for quick replacement.
- Provides consistent optical selection for repeatable readings.
Molecular Devices SpectraDrop Micro-Volume Microplate Kit, High-Throughput, 2/4 μL
The SpectraDrop MV HTS Kit is a high-throughput micro-volume microplate kit for low-volume quantitation of DNA, RNA, and protein using 2 μL and 4 μL sample volumes. The kit provides an adapter, multiple low-volume slides in 24- and 64-well formats, top cover slides with defined path lengths, and a protective carrying case for storage and transport.
- High-throughput micro-volume solution for low-volume measurements (2 μL and 4 μL)
- Includes adapter, five 24-well bottom slides, five 64-well bottom slides, and multiple top cover slides
- Teflon-coated bottom slides for precise droplet placement and easy cleaning
- Top slides with side spacers to control pathlength and reduce evaporation
- Compatible with absorbance and fluorescence detection for DNA, RNA, and protein quantitation
- Supplied in a protective carrying case for storage and transport
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Total ErbB3 (HER3) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Total ErbB3 (HER3) Detection Kit, 100 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total RAB10 Detection Kit, Control Lysate
HTRF Human & Mouse Total RAB10 Detection Kit, Control Lysate